mouse monoclonal anti human complement factor bb antibody  (Bio-Rad)

Journal: Frontiers in Immunology

Article Title: Alternative Complement Pathway Is Activated and Associated with Galactose-Deficient IgA 1 Antibody in IgA Nephropathy Patients

doi: 10.3389/fimmu.2021.638309

Figure Lengend Snippet: Glomerular complement factor Bb and C3c deposits were detected in IgAN patients. (A, B) Mesangial positivity can be seen in the complement factor Bb and C3c stains on the glomerulus of a patients with IgA nephropathy. (C, D) No intensity can be detected in the complement factor Bb and C3c stains on the glomerulus of a patient with focal segmental sclerosis. (Magnification: 400X).

Article Snippet: For factor Bb staining, tissue sections were incubated with mouse anti-human complement factor Bb antibody (Bio-Rad, UK).

Techniques:

Journal: Frontiers in Immunology

Article Title: Alternative Complement Pathway Is Activated and Associated with Galactose-Deficient IgA 1 Antibody in IgA Nephropathy Patients

doi: 10.3389/fimmu.2021.638309

Figure Lengend Snippet: Decreased activation of alternative and terminal complement pathway and stabilized renal function after immunosuppression. (A) eGFR decreased slightly among patients who did not receive immunosuppression ( P = 0.028). (B) No change in eGFR among patients received immunosuppression. (C) UPCR tended to decrease slightly among patients who did not receive immunosuppression ( P = 0.051). (D) UPCR decreased significantly in patients who received immunosuppression ( P < 0.0001). (E) Plasma Gd-IgA 1 level tended to decrease 1 months after immunosuppression (N = 17, P = 0.0638) and 3~6 months after immunosuppression (N = 20, P = 0.0019). (F) Plasma C5a significantly decreased 1 (N = 17, P < 0.0001) and 3~6 months after immunosuppression (N = 20, P < 0.0001). (G) Plasma factor Ba level significantly decreased 1 (N = 17, P = 0.0008) and 3~6 months after immunosuppression (N = 20, P = 0.0004). UPCR, urinary protein to creatinine ratio; eGFR, estimated glomerular filtration rate.

Article Snippet: For factor Bb staining, tissue sections were incubated with mouse anti-human complement factor Bb antibody (Bio-Rad, UK).

Techniques: Activation Assay, Clinical Proteomics, Filtration

Journal: Clinical and Experimental Immunology

Article Title: Neutrophil extracellular traps can activate alternative complement pathways

doi: 10.1111/cei.12654

Figure Lengend Snippet: Detection of deposition of components of alternative complement on neutrophil extracellular traps (NETs) in vitro. Neutrophils isolated from healthy donors were primed with tumour necrosis factor (TNF)-α and incubated with 250 µg/ml anti-neutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)G. NETs induced by ANCA were identified by co-localization of DNA (blue), elastase, cit-histone H3 or myeloperoxidase (MPO) (green). Deposition of complement factor B (Bb) and properdin (red) on NETs was assessed by confocal microscopy. (a) Deposition of Bb (red) on NETs stained by DNA (blue) and elastase (green). Magnification ×400. (b) Deposition of properdin (red) on NETs stained by DNA (blue) and elastase (green). Magnification ×400. (c) Deposition of Bb (red) on NETs stained by DNA (blue) and cit-histone (green). (d) Deposition of Bb (red) on NETs stained by DNA (blue) and MPO (green). (e) Deposition of properdin (red) on NETs stained by DNA (blue) and cit-histone (green). (f) Deposition of properdin (red) on NETs stained by DNA (blue) and MPO (green). Representative images of six independent experiments are shown. (c–f) Scale bars = 10 μm.

Article Snippet: The specimens were incubated with MPO-specific rabbit monoclonal antibody (1 : 100 dilution; Abcam, Cambridge, UK), rabbit anti-elastase antibody (1 : 100 dilution; Abcam) or anti-Cit-histone H3 antibody (1 : 50 dilution; Abcam) in blocking buffer combined with mouse anti-human complement factor B (Bb) (1 : 50 dilution; Quidel, San Diego, CA, USA) or with mouse anti-human properdin (1 : 50 dilution; LifeSpan BioScience, Seattle, WA, USA) for 1 h at 37ºC.

Techniques: In Vitro, Isolation, Incubation, Confocal Microscopy, Staining